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高速AFM研究開発部門

超解像AFM研究開発部門

ニュース・セミナー・リンク

公　募

メンバー

イメージング研究部門

分子・細胞研究部門

諮問委員会

5年間の活動報告

”イメージング研究部門”

bR in response to light

walking myosin V

rotorless F1-ATPase

mica surface

water layers on mica

高速原子間力顕微鏡（高速AＦＭ)と超解像液中ＡＦＭを使って、生体分子の構造とダイナミクスを研究している。従来の方法とは異なり、高速ＡＦＭは機能している分子の構造とダイナミクスの同時記録を可能とし、それ故、どのように生体分子が働くかの直截的な理解を可能とする。また、超解像液中ＡＦＭでは、αヘリックスやβシートといったタンパク質の2次構造をも観察可能であり、タンパク質の構造と機能の関係をより詳細にべることができる。イメージング研究部門は、新メンバー3名の補充により、2011年度中に完成する予定である。

進行中の研究: どのようにして生体分子が働くかを理解するために、高速ＡＦＭを使ってダイナミックな生体分子プロセスを研究している。これまでに、アクチンフィラメントに沿って歩くミオシンVや光に応答するバクテリオロドプシンの構造変化などの動的プロセスのイメージングに成功している。ミオシンVI、ダイニン、種々の天然変性タンパク質などの他の分子の動的振る舞いの観察を現在試みている。また、超解像ＦＭ−ＡＦＭを用いて、物質表面の周りの水和層やタンパク質の２次構造を研究している。細胞に適用できる高速ＡＦＭの開発がある程度進んだときには、細胞のＡＦＭ観察も行う。

Selected Publications

T. Ando, "High-speed atomic force microscopy coming of age", Nanotechnology 23: 062001 (27 pp)  (2012).

K. Igarashi, T. Uchihashi, A. Koivula, M. Wada, S. Kimura, T. Okamoto, M. Penttilä, T. Ando, and M. Samejima, "Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface", Science 333:1279-1282 (2011).

T. Uchihashi, R. Iino, T. Ando, and H. Noji, "High-speed atomic force microscopy reveals rotory catalysis of rotorless F1-ATPase, Science 333: 755-758 (2011).

N. Kodera, D. Yamamoto, R. Ishikawa, and T. Ando, "Video imaging of walking myosin V by high-speed atomic force microscopy", Nature 468: 72-76 (2010).  (supplementary movies) (supplementary info) (News & View)

M. Shibata, T. Uchihashi, H. Yamashita, H. Kandori, and T. Ando, "Structural changes in bacteriorhodopsin in response to alternate illumination observed by high-speed atomic force microscopy",  Angewande Chemie International edition 50: 4410 –4413 (2011).

P.-E. Milhiet, D. Yamamoto., O. Berthoumieu, P. Dosset, C. Le Grimellec, J.-M.Verdier, S. Marchal, and T. Ando, "Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy", PLos One 5 (11): e13240 (8 pp) (2010).  (Movie S1, Movie S2, Movie S3) (supplementary info)

D. Yamamoto, T. Uchihashi, N. Kodera, H. Yamashita, S. Nishikori, T. Ogura, M. Shibata, and T. Ando, "High-speed atomic force microscopy techniques for observing dynamic biomolecular processes", Methods Enzymol. 475 (Part B): 541-564 (2010).

D. Yamamoto, T. Azuma, T. Uchihashi, H. Sasaki, H. Watanabe, T. Ando, and Y. Fukumori,  "Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy", Proc. Natl. Acad. Sci. USA 107: 9382-9387 (2010). (supplementary info) (supplementary movie)

S. Sugimoto, K. Yamanaka, S. Nishikori, A. Miyagi, T. Ando, and T. Ogura, "AAA+ chaperone ClpX regurates dynamics of prokaryotic cytoskeletal protein FtsZ", J. Biol. Chem. 285: 6648-6657 (2010).

M.-C. Giocondi, D. Yamamoto, E. Lesniewska, P.-E. Milhiet, T. Ando, and C. Le Grimellec, "Surface topography of membrane domains", Bioochim. Biophys. Acta.-Biomembranes 1798: 703-718 (2010)

M. Shibata, H. Yamashita, T. Uchihashi, H. Kandori, and T. Ando, "High-speed atomic force microscopy shows dynamic molecular processes in photo-activated bacteriorhodopsin", Nature Nanotechnology 5, 208 - 212 (2010).  (Supplementary Info) (Supplementary Movies) (npg asia materials highlight)

D. Yamamoto, N. Nagura, S. Omote, M. Taniguchi, and T. Ando, "Streptavidin 2D crystal substrates for visualizing biomolecular processes by atomic force microscopy", Biophys. J. 97(8): 2358–2367 (2009).

H. Yamashita, K. Voïtchovsky, T. Uchihashi, S. Antoranz Contera, J. F. Ryan, and T. Ando, "Dynamics of bacteriorhodopsin 2D crystal observed by high-speed atomic force microscopy", J. Struct. Biol. 167: 153-158 (2009).

http://www.sciencedirect.com/science/journal/10478477 (Supplementary Movie)

T. Ando, T. Uchihashi, and T. Fukuma, "High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes", Prog. Surf. Sci. 83: 337-437 (2008).

D. Yamamoto, T. Uchihashi, N. Kodera, and T. Ando, "Anisotropic diffusion of point defects in two-dimensional crystal of streptavidin observed by high-speed atomic force microscopy", Nanotechnol. 19: 384009 (9 pp) (2008).  (Supplementary Movie)  

A. Miyagi, Y. Tsunaka, T. Uchihashi, K. Mayanagi, S. Hirose, K. Morikawa, and T. Ando , "Visualization of intrinsically disordered regions of proteins by high-speed atomic force microscopy", Chem. Phys. Chem. 9(13):1859-1866 (2008).  (Supplementary Movies)

T. Fukuma, Y. Okazaki, N. Kodera, T. Uchihashi, and T. Ando, "High resonance frequency force microscope scanner using inertia balance support", Appl. Phys. Lett. 92:243119 (2008).

T. Ando, T. Uchihashi, N. Kodera, D. Yamamoto, M. Taniguchi, A. Miyagi, and H. Yamashita, "Invited Review: High-speed AFM and nano-visualization of biomolecular processes", Pflügers Archiv -Eur. J. Physiol. 456:211-225 (2008). 

T. Ando, T. Uchihashi, N. Kodera, D. Yamamoto, M. Taniguchi, A. Miyagi, and H. Yamashita, "Review: High-speed atomic force microscopy for observing dynamic biomolecular processes", J. Mol. Recognit. 20:448-458 (2007).  

H. Yamashita, T. Uchihashi, N. Kodera, A. Miyagi, D. Yamamoto, and T. Ando, "Tip-sample distance control using photo-thermal actuation of a small cantilever for high-speed atomic force microscopy", Rev. Sci. Instrum. 78:083702 (5 pages) (2007).

T. Uchihashi, H. Yamashita, and T. Ando, "Fast phase imaging in liquids using a rapid scan atomic force microscope.

Appl. Phys. Lett. 89:213112 (3 pages) (2006).

N. Kodera, M. Sakashita, and T. Ando , " Dynamic proportional-integral-differential controller for high-speed atomic force microscopy", Rev. Sci. Instrum. 77(8):083704 (7 pages) (2006).

M. Yokokawa, C. Wada, T. Ando, N. Sakai , A. Yagi, S.H. Yoshimura, and K. Takeyasu , "Fast-scanning atomic force microscopy reveals the ATP/ADP-dependent conformational changes of GroEL", EMBO J. 25:4567-4576 (2006). 

T. Ando, T. Uchihashi, N. Kodera, A. Miyagi, R. Nakakita, H. Yamashita, and M. Sakashita, "High-speed atomic force microscopy for studying the dynamic behavior of protein molecules at work", Jpn. J. Appl. Phys. 45(3B):1897-1903 (2006).

T. Uchihashi, N. Kodera, H. Itoh, H. Yamashita, and T. Ando, "Feed-forward control for high-speed AFM imaging of biomolecules", Jpn. J. Appl. Phys. 45(3B):1904-1908 (2006).

T. Ando, T. Uchihashi, N. Kodera, A. Miyagi, R. Nakakita, H. Yamashita, and K. Matada, "High-speed AFM for studying the dynamic behavior of protein molecules at work", Surf. Sci. Nanotechnol. 3:384-392 (2005).   (Supplementary Data)

N. Kodera, H. Yamashita, and T. Ando, "Active damping of the scanner for high-speed atomic force microscopy", Rev. Sci. Instrum. 76: 053708 (5pages) (2005). (Supplementary Data)

T. Ando, N. Kodera, Y. Naito, T. Kinoshita, K. Furuta, and Y.Y. Toyoshima, "A high-speed atomic force microscope for studying biological macromolecules in action", Chem. Phys. Chem. 4:1196-1202 (2003).

T. Ando, N. Kodera, D. Maruyama,  E. Takai, K. Saito, and A. Toda, "A High-speed atomic force microscope for studying biological macromolecules in action", Jpn. J. Appl. Phys. 41:4851-4856 (2002).

T. Ando, N. Kodera, E. Takai, D. Maruyama,  K. Saito, and A. Toda, "A High-speed atomic force microscope for studying biological macromolecules", Proc. Natl. Acad. Sci. USA  98:12468-12472 (2001).